GHK-Cu

Quickstart Highlights

GHK-Cu is a naturally occurring copper-bound tri‑peptide (glycyl‑L‑histidyl‑L‑lysine) studied for its regenerative and protective effects across skin, connective tissue, and other organs. This educational protocol presents a once‑daily subcutaneous dosing approach using a practical dilution for clear insulin‑syringe measurements.

• Reconstitute: Add 3.0 mL bacteriostatic water (max recommended) → ~16.7 mg/mL concentration.

• Typical daily range: 1–2 mg once daily (gradual titration protocol).

• Easy measuring: At ~16.7 mg/mL, 1 unit = 0.01 mL ≈ 167 mcg on a U‑100 insulin syringe.

• Storage: Lyophilized: store at room temperature in a dark spot or in its box; after reconstitution, refrigerate at 2–8 °C (35.6–46.4 °F). Avoid repeated freeze–thaw cycles.

Dosing & Reconstitution Guide

1. Standard / Gradual Approach (3 mL = ~16.7 mg/mL)

Frequency: Inject once daily subcutaneously. This gradual schedule reaches a moderate dose (2 mg daily) while using a dilution (3.0 mL) that keeps measurements ≥6 units for accuracy. If a very low individual dose ever falls under ~10 units, consider using 50‑unit or 30‑unit insulin syringes for finer gradations.

2. Reconstitution Steps

• Draw 3.0 mL of bacteriostatic water with a sterile syringe.

• Inject the water slowly into the vial, aiming against the vial wall to avoid foaming.

• Gently swirl or roll the vial until the powder fully dissolves (do not shake vigorously).

• Refrigerate at 2–8 °C (35.6–46.4 °F), protected from light.

Supplies Needed

Plan based on an 8–16 week daily protocol with gradual titration.

• Peptide Vials (GHK-Cu, 50 mg each):

  • 8 weeks ≈ 2 vials

  • 12 weeks ≈ 3 vials

  • 16 weeks ≈ 5 vials

  
  

• Insulin Syringes (U‑100):

  • Per week: 7 syringes (1/day)

  • 8 weeks: 56 syringes

  • 12 weeks: 84 syringes

  • 16 weeks: 112 syringes

    
    

• Bacteriostatic Water (10 mL bottles): Use ~3.0 mL per vial for reconstitution.

  • 8 weeks (2 vials): 6 mL → 1 × 10 mL bottle

  • 12 weeks (3 vials): 9 mL → 1 × 10 mL bottle

  • 16 weeks (5 vials): 15 mL → 2 × 10 mL bottles

    
    

• Alcohol Swabs: One for vial stopper + one for skin per injection.

  • Per week: 14 swabs (2/day)

  • 8 weeks: 112 swabs → recommend 2 × 100‑count boxes

  • 12 weeks: 168 swabs → recommend 2 × 100‑count boxes

  • 16 weeks: 224 swabs → recommend 3 × 100‑count boxes

Protocol Overview

Concise summary of the once‑daily regimen.

• Goal: Support tissue repair, skin rejuvenation, and healthy regeneration over time.

• Schedule: Daily subcutaneous injections for 8–12 weeks (extend to 16 weeks if needed).

• Dose Range: 1–2 mg daily with gradual upward titration.

• Reconstitution: 3.0 mL per 50 mg vial (~16.7 mg/mL) for flexible, accurate dosing.

• Storage: Lyophilized vials frozen; reconstituted solution refrigerated; avoid refreezing.

Dosing Protocol

Suggested daily titration approach.

• Start: Begin at ~1.0 mg daily; increase by ~0.5 mg every 1–2 weeks as tolerated.

• Target: Reach ~2.0 mg daily by Weeks 5–6 and maintain through Week 12 if beneficial.

• Frequency: Once per day (subcutaneous injection).

• Cycle Length: 8–12 weeks; an extended course up to 16 weeks can be considered.

• Timing: Inject at a consistent time each day; rotate injection sites to allow local recovery.

‼️ Important Notes

Practical considerations for consistency and safety.

• Use a new sterile insulin syringe for each injection and dispose of needles in a proper sharps container.

• Rotate injection sites (abdomen, thigh, upper arm) to minimize local irritation or tissue changes over time.

• Inject slowly and steadily; after injection, wait a few seconds before withdrawing the needle to prevent leakage.

• Maintain a log of daily dose and injection site to ensure consistent rotation and monitor your regimen.

General subcutaneous injection guidance (for educational purposes).

• Clean the vial’s rubber stopper and your chosen injection site with alcohol swabs; let them air-dry.

• Using an insulin syringe, pinch a fold of skin and insert the needle at a 45–90° angle into the subcutaneous tissue (fat layer).

• Do not aspirate (no need to pull back on the plunger) for subcutaneous injections. Inject the peptide slowly and steadily.

• After injection, dispose of the syringe safely. Rotate injection sites (abdomen, thighs, upper arms) to prevent irritation or scar tissue build-up.

Potential Benefits & Side Effects

GHK-Cu plays a physiological role in healing and tissue remodeling. Preclinical studies demonstrate that it accelerates wound closure and enhances tissue repair (including better healing of diabetic wounds with reduced inflammatory cytokines). Unlike large growth factors, GHK-Cu works by broadly modulating gene expression to promote regeneration (upregulating collagen synthesis, angiogenesis, and anti-inflammatory pathways). It has even shown hair growth–promoting effects comparable to conventional therapies, yet with minimal side effects observed. Findings from research and clinical observations:

• Supports enhanced wound healing, collagen production, and overall skin/tissue regeneration (noted in animal studies and cosmetic human trials).

• Exhibits broad protective effects (anti-inflammatory, anti-oxidant) and has been explored for diverse applications (from skin repair to lung health) with a favorable safety profile in studies.

• Generally well tolerated; mild injection-site redness or itching can occasionally occur. (Use is typically contraindicated in individuals with copper metabolism disorders such as Wilson’s disease.)

Important Note

This content is intended for educational purposes only and is not medical advice, diagnosis, or treatment.

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