BPC-157

Quickstart Highlights

BPC-157 (pentadecapeptide) is widely studied preclinically for cytoprotection, angiogenesis, and tissue repair mechanisms. Human dosing standards are not established; the schedules below reflect commonly referenced research practices, not medical advice.

• Reconstitute: Add 3.0 mL bacteriostatic water (max recommended) → ~1.67 mg/mL concentration.

• Typical daily range: 200–300 mcg once daily (Standard) or 250 mcg twice daily (Advanced)

• Easy measuring: At ~1.67 mg/mL, 1 unit = 0.01 mL ≈ 16.7 mcg on a U‑100 insulin syringe. 200–300 mcg → 12–18 units

• Cycle length: 4–8 weeks depending on results

• Storage: Lyophilized: store at room temperature in a dark spot or kept in its box; after reconstitution, refrigerate at 2–8 °C (35.6–46.4 °F). Avoid repeated freeze–thaw cycles.

Dosing & Reconstitution Guide

1. Standard / Gradual Approach (3 mL = ~1.67 mg/mL)

Why 3 mL? Using the largest reconstitution volume helps keep per-injection measurements at or above ~10 units for accuracy on 100-unit syringes. If a very low individual dose ever falls under ~10 units, consider using 50‑unit or 30‑unit insulin syringes for finer gradations.

Reconstitution Steps

• Draw 3.0 mL of bacteriostatic water with a sterile syringe.

• Inject the water slowly into the vial, aiming against the vial wall to avoid foaming.

• Gently swirl or roll the vial until the powder fully dissolves (do not shake vigorously).

• Refrigerate at 2–8 °C (35.6–46.4 °F), protected from light.

2. Advanced / Aggressive Approach (2 mL = 2.5 mg/mL)

Using 2.0 mL yields 25 mcg per unit, keeping twice-daily injections at practical volumes while staying above ~10 units per injection.

Reconstitution Steps

• Draw 2.0 mL of bacteriostatic water with a sterile syringe.

• Inject the water slowly into the vial, aiming against the vial wall to avoid foaming.

• Gently swirl or roll the vial until the powder fully dissolves (do not shake vigorously).

• Refrigerate at 2–8 °C (35.6–46.4 °F), protected from light.

Supplies Needed

Approximate quantities for common 4–8 week designs.

• Peptide Vials (BPC-157 5mg):
  • 6 wks Standard ≈ 2 vials (≈9.8 mg total)
  • 8 wks Standard ≈ 3 vials (≈14 mg)
  • 8 wks Advanced (2×/day) ≈ 6 vials (≈28 mg)

  
  

• Insulin Syringes:
  • 6 wks 1×/day ≈ 42
  • 8 wks 1×/day ≈ 56
  • 8 wks 2×/day ≈ 112

  
  

• Bacteriostatic Water (10 mL bottles): 2–3× 10 mL bottles (reconstitute each vial with 2–3 mL)

  
  

• Alcohol Swabs: One for vial stopper + one for skin per injection.

  • Per week: 14 swabs (2/day)

  • 8 weeks: 112 swabs → recommend 2 × 100‑count boxes

Protocol Overview

Concise summary of the once‑daily regimen.

• Goal: Promotes muscle and tendon repair, support gut and stomach lining health, reduces inflammation and pain.

• Schedule: Daily subcutaneous injections for 4–8 weeks

• Dose Range: 200–300 mcg once daily (3 mL dilution keeps ≥10 units). For advanced dosing, 250 mcg twice daily (2 mL dilution → 10 units per injection).

• Cycle: 4–8 weeks depending on study aim and observations

• Reconstitution: 3.0 mL per 5 mg vial for flexible, accurate dosing. (or 2 mL for the advanced approach).

• Storage: Lyophilized vials frozen; reconstituted solution refrigerated; avoid refreezing.

‼️ Important Notes

Practical considerations for consistency and safety.

• Use a new sterile insulin syringe for each injection and dispose of needles in a proper sharps container.

• Rotate injection sites (abdomen, thigh, upper arm) to minimize local irritation or tissue changes over time.

• Inject slowly and steadily; after injection, wait a few seconds before withdrawing the needle to prevent leakage.

• Maintain a log of daily dose and injection site to ensure consistent rotation and monitor your regimen.

General subcutaneous injection guidance (for educational purposes).

• Clean the vial’s rubber stopper and your chosen injection site with alcohol swabs; let them air-dry.

• Using an insulin syringe, pinch a fold of skin and insert the needle at a 45–90° angle into the subcutaneous tissue (fat layer).

• Do not aspirate (no need to pull back on the plunger) for subcutaneous injections. Inject the peptide slowly and steadily.

• After injection, dispose of the syringe safely. Rotate injection sites (abdomen, thighs, upper arms) to prevent irritation or scar tissue build-up.

Potential Benefits & Side Effects

Key mechanisms reported in preclinical literature:

• Angiogenesis: Engagement of VEGFR2–Akt–eNOS signaling; pro-angiogenic effects in ischemia and tube-formation assays

• NO System Modulation: Endothelium-dependent vasodilation influenced by nitric-oxide pathways

• Cytoprotection: Protection of GI mucosa and multi-organ tissues consistent with “cytoprotection” frameworks

• Tendon/Soft-Tissue Models: Accelerated healing and cell migration in tendon and myotendinous junction studies

Potential side effects:

• Injection-site irritation or erythema

• Regulatory and anti-doping compliance requirements

  
  

Important Note

This content is intended for educational purposes only and is not medical advice, diagnosis, or treatment.

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